26-hydroxylation of 5 beta-cholestane-3 alpha,7 alpha,12 alpha-triol by isolated nonparenchymal cells and hepatocytes from rat liver
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چکیده
منابع مشابه
26 - Hydroxylation of SP - cholestane - 3 a , 7 a , 1 2 a - triol by isolated nonparenchymal cells and hepatocytes from rat liver
The ability of isolated nonparenchymal and parenchymal rat liver cells to metabolize 5&cholestane-3a,7a, 12atriol and other bile acid intermediates has been investigated. Incubation of nonparenchymal cells with 5&cholestane3a,7a, 12a-triol resulted in the formation of one more polar product identified as 5&cholestane-3a,7a, 12a,26-tetrol. The formation was linear with time up to 2 hr and with t...
متن کاملCerebrotendinous xanthomatosis: a defect in mitochondrial 26-hydroxylation required for normal biosynthesis of cholic acid.
Oxidation of side chain of 5 beta-cholestane-3 alpha,7 alpha,12 alpha-triol was studied in a patient with cerebrotendinous xanthomatosis (CTX) and in control subjects, using various subcellular fractions of liver homogenate and a method based on isotope dilution-mass spectrometry. In the control, 5 beta-cholestane-3 alpha,7 alpha,12 alpha-triol was converted into 5 beta-cholestane-3 alpha,7 alp...
متن کاملDemonstration of 26-hydroxylation of C27-steroids in human skin fibroblasts, and a deficiency of this activity in cerebrotendinous xanthomatosis.
26-Hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol and other C27-steroids was demonstrated in cultured skin fibroblasts from healthy individuals. Activities in skin fibroblasts were approximately 5-10% of those previously found in human liver homogenates, and were inhibited by CO. The apparent Km was lowest for 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol (1.3 mumol/li...
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IN Part 1 [Haslewood, 1939], the isolation from ox liver of oc-7-hydroxycholesterol was reported. Since that time, a '/3-7-hydroxycholesterol' has been mentioned as isolated from hog liver [MacPhillamy, 1940], and /3-7-hydroxycholesterol has been obtained from pregnant mares' serum [Wintersteiner & Ritzmann, 1940]. Wintersteiner & Bergstrom [1941] report further that cholesterol can be converte...
متن کامل25-hydroxylation of C27-steroids and vitamin D3 by a constitutive cytochrome P-450 from rat liver microsomes.
A constitutive cytochrome P-450 catalyzing 25-hydroxylation of C27-steroids and vitamin D3 was purified from rat liver microsomes. The enzyme fraction contained 16 nmol of cytochrome P-450/mg of protein and showed only one protein band with a minimum molecular weight of 51,000 upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified cytochrome P-450 catalyzed 25-hydroxylatio...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1982
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38038-x